Since its incorporation, Artron BioResearch Inc. has been using mouse hybridoma technology to produce monoclonal antibodies. Should you desire custom monoclonal antibodies to be developed against a provided antigen, our experienced and talented research scientists will meet with you to discuss such possibilities. To ensure customer satisfaction, we will thoroughly discuss each step of the process with you. The final product; frozen ascites/sera, cryopreserved cell lines, and monoclonal antibodies belongs to you, the client.
Due to antigens' natural variability and complex processes, pricing may vary between cases. Please contact us for more details.
Mice will be immunized with the client-specified antigen by either a client-specified method or by Artron’s recommended method. To improve the overall success, Artron can assist with the purification of the client-specified antibody. Higher levels of purity usually improve the overall success of generating useful antibodies.
Upon receiving your request and determination of the antigen's nature, a senior scientist will establish the immunization process details. The process details will then be reported to you for approval (conjugation may be required for certain antigens). Mice sera will be checked periodically for specific antibodies using assay systems such as ELISA, Western Blotting, or a specified assay. Sera will be made available for your examination before Artron advances to the next step: cell fusion.
Cell Fusion and Screening (Clonal Selection):
Once you have approved the sera, the mice splenocytes are fused with myeloma cells. The clones are then screened with ELISA. Clones that yield a positive ELISA test result will be cultured. You will determine the number of clones to be kept/cultured based on how many monoclonal antibodies are needed. If desired, hybridoma supernatant will be made available to you for further testing.
Cloning: Dilution cloning will be performed on the selected clones to ensure antibody activity and monoclonality.
Once antibody activity and monoclonality are ensured, cells will be injected into mice for ascites production. Ascites will then be collected and frozen. If desired, a sample can be provided to you for examination.
Once ascites collection is completed, our researchers will determine the most favourable purification method to ensure the best possible combination of high purity and activity. A report of the proposed purification strategies and any relevant issues that might arise will be made available to you. Upon approval, technicians will proceed with the purification stage.